Presented at PAGXXV

Research area: plant_biology

Modifications to a LATE MERISTEM IDENTITY-1 gene are responsible for the major leaf shapes of Upland cotton (Gossypium hirsutum L.)

Created on 7th July 2016

Ryan J Andres; Viktoriya Coneva; Margaret Frank; John R Tuttle; Sang-Won Han; Luis F Samayoa; Baljinder Kaur; Linglong Zhu; Hui Fang; Daryl T Bowman; Marcela Rojas-Pierce; Candace H Haigler; Don C Jones; James B Holland; Daniel H Chitwood; Vasu Kuraparthy;

Manipulation of cotton canopy architecture offers the potential to maximize yield while minimizing inputs. Here we show that the major leaf shapes of cotton at the L-D1 locus are controlled by a Late Meristem Identity1-D1b (GhLMI1-D1b) gene which encodes a HD-Zip transcription factor. Okra leaf GhLMI1-D1b has a 133-bp tandem duplication in the promoter, correlated with elevated expression, while an 8-bp deletion in the third exon of normal leaf GhLMI1-D1b causes a frame-shifted and truncated coding sequence. Virus-induced gene silencing (VIGS) of GhLMI1-D1b in an okra variety was sufficient to induce normal leaf formation. An intermediate leaf shape allele, sub-okra, lacks both the promoter duplication and the exonic deletion. Our results indicate that sub-okra is the ancestral leaf shape of tetraploid cotton and normal is a derived mutant allele that came to predominate and define the leaf shape of cultivated cotton.

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