Created on 7th July 2016
Manipulation of cotton canopy architecture offers the potential to maximize yield while minimizing inputs. Here we show that the major leaf shapes of cotton at the L-D1 locus are controlled by a Late Meristem Identity1-D1b (GhLMI1-D1b) gene which encodes a HD-Zip transcription factor. Okra leaf GhLMI1-D1b has a 133-bp tandem duplication in the promoter, correlated with elevated expression, while an 8-bp deletion in the third exon of normal leaf GhLMI1-D1b causes a frame-shifted and truncated coding sequence. Virus-induced gene silencing (VIGS) of GhLMI1-D1b in an okra variety was sufficient to induce normal leaf formation. An intermediate leaf shape allele, sub-okra, lacks both the promoter duplication and the exonic deletion. Our results indicate that sub-okra is the ancestral leaf shape of tetraploid cotton and normal is a derived mutant allele that came to predominate and define the leaf shape of cultivated cotton.Show more
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