Altmetric score 101.934 (top 1%)

Author: Yinping Jiao
Research area: genomics

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The complex sequence landscape of maize revealed by single molecule technologies

Created on 20th December 2016

Yinping Jiao; Paul Peluso; Jinghua Shi; Tiffany Liang; Michelle C Stitzer; Bo Wang; Michael Campbell; Joshua C Stein; Xuehong Wei; Chen-Shan Chin; Katherine Guill; Michael Regulski; Sunita Kumari; Andrew Olson; Jonathan Gent; Kevin L Schneider; Thomas K Wolfgruber; Michael May; Nathan Springer; Eric Antoniou; Richard McCombie; Gernot G Presting; Michael McMullen; Jeffrey Ross-Ibarra; R. Kelly Dawe; Alex Hastie; David R Rank; Doreen Ware;

Complete and accurate reference genomes and annotations provide fundamental tools for characterization of genetic and functional variation. These resources facilitate elucidation of biological processes and support translation of research findings into improved and sustainable agricultural technologies. Many reference genomes for crop plants have been generated over the past decade, but these genomes are often fragmented and missing complex repeat regions. Here, we report the assembly and annotation of maize, a genetic and agricultural model organism, using Single Molecule Real-Time (SMRT) sequencing and high-resolution genome map. Relative to the previous reference genome, our assembly features a 52-fold increase in contig length and significant improvements in the assembly of intergenic spaces and centromeres. Characterization of the repetitive portion of the genome revealed over 130,000 intact transposable elements (TEs), allowing us to identify TE lineage expansions unique to maize. Gene annotations were updated using 111,000 full-length transcripts obtained by SMRT sequencing. In addition, comparative optical mapping of two other inbreds revealed a prevalence of deletions in the region of low gene density region and maize lineage-specific genes.

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