Included in crispr, rnaseq, singlecell_rna_sequencing themes

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Author: Paul Datlinger

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Pooled CRISPR screening with single-cell transcriptome read-out

Created on 29th October 2016

Paul Datlinger; Christian Schmidl; Andre F Rendeiro; Peter Traxler; Johanna Klughammer; Linda Schuster; Christoph Bock;

CRISPR-based genetic screens have revolutionized the search for new gene functions and biological mechanisms. However, widely used pooled screens are limited to simple read-outs of cell proliferation or the production of a selectable marker protein. Arrayed screens allow for more complex molecular read-outs such as transcriptome profiling, but they provide much lower throughput. Here we demonstrate CRISPR genome editing together with single-cell RNA sequencing as a new screening paradigm that combines key advantages of pooled and arrayed screens. This approach allowed us to link guide-RNA expression to the associated transcriptome responses in thousands of single cells using a straightforward and broadly applicable screening workflow.

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